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Alternative versus gold standard method for the typing of vancomycinresistant Enterococcus faecium: semi-automated rep-PCR system

Gulsen Hazirolan, Sumeyra Savas, Alper Karagoz, S. Ahmet Bayik, Ipek Mumcuoglu, Neriman Aksu, Riza Durmaz

Vancomycin-Resistant Enterococcus faecium (VRE) are significant nosocomial pathogens with limited treatment options. Vancomycin-Resistant Enterococcus faecium (VRE) has become possible transfer of vancomycin resistance to more virulent pathogens. The current study examined the detection of inhospital distribution and the spread of Vancomycin-Resistant Enterococcus faecium (VRE), in addition to the comparison of the availability of the methods of Pulsed Field Gel Electrophoresis (PFGE) and the Rep-PCR DiversiLab method in the detection of the clonal association between Vancomycin-Resistant Enterococcus faecium (VRE) isolates. Twenty-two humans and twenty-two environmentally originated isolates isolated from different wards were included in the study. A total of four clones were identified by the Rep-PCR DiversiLab during the evaluation after the finger analysis. Six isolates were found to belong none of the clones. On the other hand, Pulsed Field Gel Electrophoresis (PFGE) typing yielded five Pulsed Field Gel Electrophoresis (PFGE) including a total of 47 clonally related isolates and one unique isolate. As a result of one-to-one comparisons of the PFGE DNA patterns of a total of 48 species. The fact that the isolates were isolated from different clinics and samples suggested that the majority of Vancomycin-Resistant Enterococcus faecium (VRE) isolates in the hospital environment spread through cross contamination. The Pulsed Field Gel Electrophoresis (PFGE) method had greater reliability and differentiating capacity according to the comparison of both methods using epidemiological data.