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Biochemical effect of various anti-diabetic therapies on obesity and insulin resistance induced by monosodium glutamate in male rats

Sherin. M. Khodier

Background: This research aims to summarize the metabolic changes in male obese rats induced by MSG and study the possible effect of Metformin, DPP4 inhibitor (Janumet), liraglutide (Victoza) on improvement this metabolic changes through the determination of the following parameters (Triacylglycerol, Cholesterol, fasting blood glucose, Glucagon, Insulin, HbA1c, Leptin, Resistin and inflammatory Cytokines (TNF- and Interleukin-6), beside calculated HOMA- IR which contribute in obesity and their complication. Methods: 60 male rats divided into control group A (n=12) received distilled water, and MSG group B (n=48) administered orally with MSG at a dosage (15 mg per kg body weight) for three months. After confirmation of obesity, MSG administrated rats were began the treatment strategy and divided into 2 sections 1st section (n=24) continued administration with MSG and 2nd section (n=24) continued without administration of MSG, and each section contains four groups each group (n=6), non-treated MSG obese rat. Victoza, Janumet, and Metformin, after a month from beginning the treatment, body weight and the previous parameters were measured. Results were articulated as mean ± SE and every one statistical comparison was created by a one-way ANOVA experiment followed by Duncan's post hoc multi-range test using SPSS, version 20.0. Results: MSG obese rats were shown a marked rise in body weight average, Triacylglycerol, Cholesterol, Fasting sugar - HBA1c - Insulin, HOMA-IR, leptin, resistin, and pro-inflammatory Cytokines). While the lifestyle modification model and all treated groups showed a reduction in average B.W and a significant improvement in Triacylglycerol, Cholesterol, FBS, HbA1c, insulin, HOMA-IR, leptin, resistin, TNF-α, and IL-6. Conclusion:This study indicates that lifestyle modification and anti-diabetic drugs used (Victoza, Janumet, and metformin) were improved carbohydrate and lipid metabolism, insulin sensitivity and pro-inflammatory cytokines levels, Thus it can be used for prevention occurrence of T2DM in cases suffer from obesity and insulin resistance.