Абстрактный

Bioengineered human endometrium in vitro- A novel model for implantation research

Patki Satish M, Patki Shweta S, Patil Rajendra

Objective: To develop the tissue culture technique for 3 dimensional creation of bioengineered human endometrium in a culture dish for morphological and molecular studies of its components. Settings and design: This is a laboratory based observational study during the period of 2 years from May 2014 to May 2016. Patients/ Intervention: 100 samples of the endometrium were obtained by the procedure of single biopsy in a proliferative phase of the cycle. The cases of uterine and endometrial pathologies were excluded. The cells were trypsinised and cultured on matrigel. The culture was supplemented with sequential estradiol and progesterone. Main outcome measures: Development of glanduloepithelial, stromal and vascular components of the endometrium in the culture dish. Induction of pinopodes after progestogenic stimulation on estradiol primed endometrium. Results: The stromal cells were observed like fibroblasts at the floor of culture dish while the glanduloepithelial components were observed at the surface. The epithelial cells were differentiated in to pinopodes between day 3 to 5 exposure of progesterone which are the hallmarks of the process of implantation. Conclusion: The human endometrium can be bioengineered in the culture dish, which can be a novel model for implantation research. It can avoid the need of repeated invasive endometrial biopsies and replace studies based on animal models.