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Chemistry Congress 2019 Enzymatic Degradation Comparison of Silk Fibroin Hydrogel (SFH), Silk Xanthan Hydrogel (SXH) and Silk Silver-Nanoparticles Hydrogel (SSH) for Drug Delivery Systems (DDS) Applications

Ibrahim Omodamilola Omoyayi

 Drug delivery systems (DDS) reduces side effects as oppose to conventional by targeting and releasing its dosage at specific site in the body. Successful DDS application requires a stable drug  delivery mechanism of the drug carrier. Hydrogel based biomaterial has been used over the years to demonstrate successful biomedical application in vitro. This is due to their high biocompatibility and less cytotoxicity. Silk fibroin (SF) has proven to be an excellent choice of hydrogel based biomedical application, its three-dimensional stable network of polymers allows its swelling in large amount of water. In addition, it also degrades continuously overtime which makes it highly suitable as a smart drug vehicle. In an attempt to optimize the properties of silk fibroin, several methodological approaches to silk fibroin hydrogel preparation was adopted and evaluated. The approach here was to regenerate silk fibroin solution, process it by crosslinking with silver nanoparticles (AgNPs) and xanthan solution respectively. Silk Fibroin solution stored at 4°C was dried at room temperature for 3 days to obtain Silk Fibroin Hydrogel (SFH). Silk fibroin was cross-linked with Xanthan to obtain a stable hydrogel (SXH) at its optimum pH and temperature. Lastly, AgNPs was crosslinked with SF to produce SSN. All Hydrogels samples were freeze-dried into scaffolds, Essential Biomaterial Characterization and Enzymatic degradation analysis was carried out on all hydrogels at α=0.05 level.

Introduction:

Drugs have been used extensively to improve health care. Health care technology has shi? to the practice of drug delivery systems (DDS) in the past few decades to directly deliver treatment at specific targeted while also minimizing side e??ect as oppose the conventional. ?is great advancement has been implemented to several treatment therapy and even greater changes are anticipated in the near future. Biomedical engineers have contributed greatly to the understanding of the biochemical mechanism of invaded tissue cells with its corresponding physiological barriers to e?ciently deliver drug at this site. ?e biosynthesis of smart drug delivery using di??erent biosynthetic techniques, polymer manipulation to form a stable hydrogel has picked the interest of several researchers in the field. DDS monitors and controls drug release dosage at specific site location. Silk fibroin obtained from Bombyx mori has been studied over the decades for hydrogel due to its excellent biocompatibility in vitro biomedical applications [1,2]. Its protein polymeric structures allows for its excellent biocompatibility with tissue cells. ?is polymeric network known as SILK I or SILK II possesses great mechanical quality excellent for bearing load which makes silk adequate for as a drug carrier, this diverse range of properties allows are broader span of silk fibroin as potential biomaterial. Xanthan is inhumane to a wide scope of temperature, pH and electrolyte fixations [3,4]. It likewise shows high shear steadiness across this physiological conditions. ?e presence of silver nanoparticles in silk fibroin hydrogel has been demonstrated in vivo to reduce bacterial contamination. ?e study therefore aims to determine the optimum processing condition for a silk-induced hydrogel for drug delivery system.