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CTHRC1 promotes the proliferation and invasion of prostate cancer cells by regulating the activity of Wnt/PCP signalling pathway

Chenguang Zhang, Wei Zhong, Weihua Huang

This study aims to observe whether Collagen Triple Helix Repeat Containing-1 (CTHRC1) can promote the proliferation, migration and invasion of prostate cancer cells by regulating the activity of the Wnt/PCP signalling pathway. CTHRC1 expression in prostate cancer tissues and cells was detected by RT-PCR. PcDNA3.1-CTHRC1 and CTHRC1 siRNA was transfected into prostate cancer PC3 cells using electroporation method. The effect of CTHRC1 on the proliferation of prostate cancer PC3 cells was detected by clone formation assay; the effect of CTHRC1 on the migration of prostate cancer PC3 cells were detected by wound healing assay and Transwell test; and the effect of CTHRC1 on the activity of signalling pathway was detected by dual luciferase reporter assay. RT-PCR results revealed that CTHRC1 expression was significantly higher in prostate cancer tissue compared with normal tissues. Western blot revealed that transfection with CTHRC1 could significantly promote CTHRC1 expression in prostate cancer cells. Clone formation assay revealed that transfection with CTHRC1 could significantly promote the proliferation of prostate cancer cells. Wound healing assay and Transwell test revealed that transfection with CTHRC1 could significantly promote the migration of prostate cancer cells. Dual luciferase reporter assay revealed that CTHRC1 protein could significantly activate Wnt/PCP signalling pathway in prostate cancer PC3 cells. CTHRC1 could promote the proliferation, migration and invasion of prostate cancer cells by regulating the activity of the Wnt/PCP signalling pathway. This may be the mechanism by which CTHRC1 is involved in the occurrence and development of prostate cancer.