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Detection of tetracycline veterinary drug residues in Egyptian poultry meat by high performance liquid chromatography.

Aman IM, Ahmed HF, Mostafa NY, Kitada Y, Kar G

The aim of the study is to detect tetracyclines veterinary drug residues in poultry meat. In this study one hundred and sixtybroiler chicken carcasses each of breast muscle, thigh muscle, and liver and kidney samples were collected from Kafrelsheikh city during the period from July 2009 to August 2010. Collected samples were examined for Tetracyclines TCs (Oxytetracycline - OTC and Tetracyline -TC) veterinary drug residues using HPLC technique. The extraction of both OTC and TC from chicken samples was carried out with a mobile phase consisting of methanol: acetonitrile: 0.01 M oxalic acid dihydrate (5:18:77 v/v/v) in isocratic mode. Detection carried out by UV detector at wavelength 360 nm. The chromatographic separation was achieved using Inertsil ODS-3 column with a flow rate 1 mL/min. As well as oxytetracycline and tetracycline antibiotics showed an average retention time of 3.33 min and 4.15 min, respectively. The recovery studies of OTC drug at different concentrations (0.1, 0.2, 1.0 and 2.0 μg/g) were 86.97%, 83.39% and 83.90% for breast muscle, liver and kidney samples respectively. While TC drug showed average recovery percentages at the previous concentrations of 77.56%, 74.63% and 72.45% for chicken muscle, liver and kidney samples respectively. Residues of OTC were detected in 28.75% and 75% of breast and thigh muscles respectively, while all (100%) liver and kidney samples had OTC residues. Only 8.69%, 15.83%, 45% and 51.25% of breast muscle, thigh muscle, and liver and kidney samples, respectively exceeded the MRL set by FAO/WHO 2010. Residues of TC were detected in 5.0%, 12.5%, 13.1% and 5.0% of breast muscle, thigh muscle, liver and kidney samples respectively. None of the examined liver and kidney samples contained TC quantities above the MRL while 50% each of breast and thigh muscles contained tetracycline residues above the MRL set by FAO/WHO 2010