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Development of strategies for battle against COVID-19 (a mini-project)

Iskra V Sainova

Statement of the Problem: Despite of the key importance the immunity, the activated immune reaction, including by vaccines, could cause unwished effects, for instance in cases as allergies, auto-immune diseases and disorders, as well as cardio-vascular pathologies. Toxic side effects of the chemical anti-viral preparations should also be taken in consideration. So, the main goal is connected with suppression of the cellular penetration and/or replication of COVID-19, both in vitro and in vivo, by RNA-interference of respective viral genes with appropriate siRNAs. Methodology & Theoretical Orientation: In vitro-incubated cells should be inoculated with viral strain with RNA-genome (if is possible, belonging to Coronaviridae family), which should then be treated with appropriate siRNAs against genes in the viral RNA-genome, necessary about viral penetration in the cell and/or viral replication. Subsequent evaluation on the in vivo-influence of the tested siRNAs on appropriate experimental animals, infected with the same RNA-viral strain, should further be performed.