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Differential Techniques Used for Detection of Cryptosporidium Oocysts in Stool Specimens

Sumaira Shams, Saima Khan, Asar Khan, Iftikhar Khan, Muhammad Ijaz, Atta Ullah,

Cryptosporidium is a coccidian protozoon belongs to phylum Apicomplexa, and an important etiologic agent of diarrheal disease in both immune-competent and immune-compromised hosts. There are many diagnostic techniques available for detecting and diagnosing of cryptosporidium spp. The microscopic methods are used in many studies but they are not reliable as compared to the molecular and serological methods. Most of these methods are time consuming and need expert skills to be carry out for clinical investigations. The detection and diagnosis of cryptosporidiosis in Pakistan is narrowed to major research laboratories and research centers therefore, it does not feature in protocols for routine investigations in many clinical laboratories. This review deals with the differential currently available diagnostic methods and their utilization as a standard for implementation of routine clinical diagnosis against cryptosporidium infection.