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Evaluation of IFAT reliability in diagnosing Sarcocystis spp. in Egyptian water buffalo (Bubalus bubalis)

Adel Aziz AR, Zakaib Ali FA, Salman D

The aim of the present work is to evaluate the diagnostic reliability of the indirect immunofluorescence antibody technique (IFAT) in Sarcocystis spp. from Egyptian water buffaloes (Bubalus bubalis) and determine the current Sarcocystis spp. incidence rates in Egyptian water buffalo in the Sohag area. Using traditional and immunological strategies, in 2017, a total of 145 samples were collected from neighborhood abattoirs in the territory from the esophagus, diaphragm, tongue, and masseter muscles, S. buffalonis, S. leavenii and S. fusiformis were recognized and the infestation rates were determined to be 26.9% by gross examination at the abattoir, 52.4% by microscopic examination, and 64.8% by IFAT. It was concluded that traditional gross exam at the abattoirs is not adequate to present safe meat for human utilization and Sarcocystis examination must be consolidated with other serological tests such as IFAT. In this study, IFAT found to have moderate specificity and high sensitivity in diagnosing sarcocystosis, in contrast, it had a mild capacity to predict the presence of macroscopic cysts, and it exhibited high efficacy in predicting the absence of the microscopic cysts.