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Expression of Nogo-A and Ngr after focal cerebral ischemic preconditioning in rates with cerebral ischemia-reperfusion

Zifeng Dong, Shunai Jin, Xiaoling Yuan, Tonghang Zhao

Objective: To observe the expression of Nogo-A and NgR in rats' brain after focal cerebral ischemic preconditioning.

Methods: We built cerebral ischemic preconditioning models and cerebral ischemia-reperfusion models, and assigned healthy male SD rats into sham operation group, ischemia-reperfusion group, and ischemic preconditioning group. Each group were divided into three subgroups including subgroup 1 d, subgroup 2 d, and subgroup 7 d. The time of preconditioning was 10 min. The immunohistochemical method and RT-PCR method were used for detecting the expression of Nogo-A and NgR and the expression of NgR mRNA in the ischemic hippocampus of rats respectively.

Results: Compared with the sham operation group, the expression of Nogo-A and NgR in the ischemiareperfusion group raised at d 1, 2 and 7; after the intervention of cerebral ischemic preconditioning, the expression of Nogo-A and NgR at d 1, 2, and 7 decreased much more than that in the ischemiareperfusion group (P<0.05).

Conclusion: The intervention of preconditioning for focal cerebral ischemia can reduce the expression of Nogo-A and NgR in the brain of rats which indicates it may enhance the neuroprotective effect of brain ischemic tolerance.