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New roles in mitochondria are revealed by the NEK10 interactome and depletion

Chunyan Zhong

Different facets of the cell cycle are controlled by members of the NEK protein kinase family (also known as NIMA-related kinases). According to reports, NEK10 participated in the upkeep of the G2/M checkpoint after being exposed to ultraviolet radiation. On the other hand, the functions of the NEK1, NEK5, NEK2, and NEK4 proteins have been connected to mitochondria. HEK293T cells were transfected with FLAG-NEK10 or FLAG-empty vector before being given Zeocin or not. Trypsin digestion of the proteins co-precipitated with FLAG constructs was followed by LC-MS/MS proteomic analysis. Following the retrieval of proteomic data, the Integrated Interactome System was used to analyse it, and Cytoscape was used to assemble differentially expressed proteins into protein networks and assign them to Gene Ontology biological processes. Two durable Nek10 silenced HeLa cell clones were produced for functional, cellular, and molecular studies.