Абстрактный

Regulation roles of lncRNA SPRY4-IT1 on the growth and migration of osteosarcoma

Boming Zhao , Kebin Liu, Xiaobin He, Jiyuan Yang, Yang Yi, Lin Cai

Objective: LncRNA SPRY4-IT1 usually plays an oncogenic role in various cancers. However, the function of SPRY4-IT1 in Osteosarcoma (OS) still hasn’t been reported. Our study aimed to investigate the regulation roles of SPRY4-IT1 in the OS.

Methods: OS tissue and the adjacent normal tissue were collected from 175 patients with OS. SPRY4- IT1 in OS cells was silenced by siRNA interference. The expression of SPRY4-IT1, Cyclin D1, Matrix Metalloproteinase-2 (MMP-2), Matrix Metalloproteinase-9 (MMP-9) and WNT/β-catenin signaling pathway-related factors-Wnt3 and Wnt5b in OS tissues and cells were detected by qRT-PCR and/or western blot. Effect of SPRY4-IT1 knockdown on growth, colony formation, migration and invasion of OS cells were detected by MTT assay, colony formation assay and cell migration and invasion assay, respectively. Correlation between expression of SPRY4-IT1 and expression of Cyclin D1, MMP-2 and MMP-9 was analysed by Pearson correlation analysis.

Results: Level of SPRY4-IT1 RNA in OS tissue was significantly higher than that in normal tissue. SPRY4-IT1 knockdown significantly reduced the viability, colony formation ability, and invasion and migration ability of OS cells. SPRY4-IT1 knockdown significantly decreased the expression of Cyclin D1, MMP-2, MMP-9 and WNT/β-catenin signaling pathway-related factors Wnt3 and Wnt5b at both mRNA and protein levels. In addition, expression of SPRY4-IT1 was positively correlated with the expression of Cyclin D1, MMP2 and MMP9.

Conclusion: SPRY4-IT1 plays an important role in the progression of OS possibly by regulating the expression of Cyclin D1, MMP-2, MMP-9 and WNT/β-catenin signaling pathway-related genes.